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Table 1: Density of solutions produced by mixing Nycodenz solution ( = 1.15 g/ml) with an isoosmotic diluent Discontinuous gradients: The four-layer format of 1.139, 1.095, 1.073 and 1.049 g/ml [1,2] has been executed with 2 ml of each of the solutions in a 15 ml tube, while the 1.10, 1.075, 1.05 and 1.0375 g/ml gradient described in ref 7 comprised 10ml, 20 ml, 10 ml and 7 ml respectively in a 50 ml tube

Prog Neurobiol.1979;13:277323

Demain and Vaishnav, 2009

[22] At this point, the holoenzyme has been formed, and the active sites are in dynamic equilibrium, each alternating between the dormant and activated states (flip-flop mechanism)