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Changes in circulating histamine were examined by mass spectrometry

Hoseinzadeh A, Esmaeili S, Sahebi R, Melak AM, Mahmoudi M, Hasannia M, et al

After blocking the membrane in 5% skim milk, primary antibody was incubated over night at 4 C

23 Daniel Bovet synthesized antihistamines in 1937 and earned the Nobel Prize in Physiology or Medicine in 1957, which brought hope in the treatment of allergic diseases and has been in clinical use to this day