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Dendritic cells were expanded from bone marrow progenitors as described 61 , labelled with Cell Tracker Deep Red (Invitrogen) and seeded at a 5:1 ratio with FRCs into a matrix of 1.8 mg ml 1 Collagen I (Corning), 2.6 mg ml 1 Matrigel (Corning) and 10% FBS in alpha-MEM 62