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The protein was eluted with 10 ml wash buffer containing 200 mM imidazole, 10 M PF-05190457

This is consistent with previous study using detached leaf disks in Arabidopsis and confirms the roles of GSNOR in protecting photosynthetic apparatus at high temperature ( SlGSNOR1 in tomato did not completely eliminate the expression of GSNOR , thus the basal activity may be sufficient for growth and development in normal conditions

After 15 min, mitochondria were incubated with 1 mM NADH

The simultaneous of CU enhanced the protective percentage of AED in sub-therapeutic dose against epileptic seizures and prevented learning and memory impairments caused by seizures, while no such improvement was observed in the group using AED at lower therapeutic doses alone