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Proteins were then precipitated by addition of 500 l methanol (pre-cooled at 80 C), 400 l LCMS-grade water, and 100 l chloroform

We and others have previously identified SW837 rectal cancer and HCT116 colon cancer cells as an in vitro model of inherently radioresistant, and radiosensitive CRC, respectively ( Metformin treatment (2.5 mM and 10 mM) significantly sensitised radiosensitive HCT116 cells to 1.8 Gy radiation, when compared to vehicle control ( Figure 1F ) (relative mean surviving fraction (SF) standard error of mean (SEM)

Targeting macrophages: therapeutic approaches in cancer

By reducing glucose uptake, it may force cells to potentially utilize more fat instead