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Western blot analysis For Western blotting, Y1-BS1 cell lysates were mixed with equal volumes of 5 Laemmli sample buffer [120 mM Tris-HCl, pH 6.8, 2% SDS (w/v), 10% sucrose (w/v), and 1% 2-mercaptoethanol] and subjected to 12% SDS-PAGE

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Neuroimaging investigations reveal a reduction in 5-HT and NE release, alongside compromised mesolimbic dopaminergic reward pathways in individuals diagnosed with depression (25)

doi: 10.1016/j.taap.2007.01.011 [DOI] [PubMed] [Google Scholar] 123.Cunha-Oliveira T, Rego AC, Garrido J, Borges F, Macedo T, Oliveira CR