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doi: 10.1016/j.ctcp.2012.01.005, 46

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However, a number of studies have recently shown that WNT signaling is also critical for the initiation of differentiation 2 , as well as the maintenance of the DE marker sex determining region y-box 17 (SOX17) 10 , and indeed our recent publication has proven that activation of the WNT pathway alone can efficiently differentiate hPSCs to DE 11

Dulbeccos modified Eagles medium (DMEM) (Gibco ), Trypsin-EDTA (0,25%) (Gibco ), Fetal bovine serum (FBS) (Gibco ), and penicillin/streptomycin (Gibco ) were purchased from Biogen (Cambridge, Massachusetts, USA)