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Biotin Deficiency Symptoms No reliable lab test exists for biotin deficiencydiagnosis relies entirely on symptoms : Hair & Skin Symptoms: Hair loss (often with loss of pigmentation ) Red rash around eyes, nose, and mouth Conjunctivitis Swollen face with irregular fat distribution Neurological Symptoms: Depression and fatigue Hallucinations Numbness in hands and feet Long-term Effects: Chronic deficiency weakens the immune system , increasing risk of fungal and bacterial infections