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Description

For each biomarker (e.g., Chlamydia pneumoniae , NLRP3, caspase-1, ASC, NGSDMD, vimentin), single-channel 20 images (covering ~150 10 3 m 2 area/per image) were converted to grayscale and standardized to baseline intensity by using a histogram-based threshold in ImageJ2/Fiji

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30,31,32 In brief, for untargeted analysis, liver tissue was homogenized and subsequently metabolites extracted in a two-step procedure

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Likewise, angiogenesis plays a critical role in tumor growth, invasion and metastasis (Semela and Dufour, 2004)

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