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As elution progresses, the stationary phase begins capturing H+ from the mobile phase, triggering a pH increase in the elution fraction, which is reflected in the chromatogram

The system was controlled using MassHunter 12 software (Agilent, Palo Alto, CA, USA)

In contrast, a study identified phosphorylation of BID by JNK at T59 prevents caspase 8 cleavage, but despite the lack of cleavage this drives apoptosis (Prakasam et al., 2014)