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Initial studies on midbrain DA-like neurons were performed on human organoids derived from embryonic stem cells (Simao et al., 2015

After washing 3 times for 10 mins in PBS, slices were incubated at RT on a shaker for 2 hours in secondary antibodies: goat anti-rabbit Alexa Fluor 594 (1:1000, Invitrogen, A11037) for MBP, goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, A11034) for Iba-1 and donkey anti-mouse Alexa Fluor 594 (1:1000, Thermo Fisher, A21203) for GFAP

Another lncRNA potentially involved in a similar nuclear complex with DNA-PKcs is NEAT1, which preferentially binds to NONO and SFPQ, and like LINP1 can act as a scaffold

The obtained data showed that although the calculated association constant was comparable in all three experiments, the dissociation constant (kd) was 9 times higher when the elution buffer 2 containing EDTA was used as dissociation buffer compared to the experiment in which binding buffer was used as elution buffer