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To summarize briefly, Figure 2b clearly demonstrated that the level of HGF nitration was drastically decreased by LASSS-pretreatment as shown by Western blotting visualized by anti-nitroY mAb-positive bands normalized by total HGF -chain (third row lanes 2 and 3 and fourth row lanes 2 and 3 )

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Bradford Protein Assay for the Quantification of Total Protein LDH, caspase 3, SOD and GPx activities, the levels of DCFDA, 8-OH-dG, TBARS, DNPH, GSSG/GSH and several cytokines, and TAC were adjusted for variations in cell number using the Bradford protein assay described by Bradford ( Data Analysis The results obtained are presented as the means the standard error of measurement (SEM)