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Preparation of single cell suspension from tissue Foot skin Whole foot skin was cut into small pieces, which were digested for 45 to 60 minutes at 37C under gentle agitation in RPMI 1640 containing 0.2 mg/mL collagenase P and 0.1 mg/mL DNAse I (both Roche Diagnostics GmbH)

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differing normalization and batch effects) and possess sufficient corresponding clinical data (particularly long-term follow-up and survival status) to perform a reliable external validation of the prognostic risk score