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In all experiments, cages were randomly assigned to treatment groups: control (CON) and B12 supplemented (cyanocobalamin at 10 g/ml, CNCbl10, and 40 g/ml, CNCbl40

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After rinsing with PBS, all sections were incubated at room temperature for 2 h with IgG goat anti-rabbit (Thermo Fisher Scientific, Alexa Fluor 488) diluted 1:200 in 0.01 M PB containing 1% BSA and 0.3% Triton-X100