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Bromelain can be found in all portions of the pineapple plant ( Ananas comosus ), but the stem is the most typical industrial source, due to its accessibility after harvesting the fruit

To estimate the effect of selenium, sodium selenite (Sigma-Aldrich, USA) in the concentration from 1 pM to 1 nM was supplemented to the medium for 3 days starting from the 5th day of differentiation

Scientific References Journal of Endocrinology (1995) "Growth hormone-releasing peptide-2 (GHRP-2), like ghrelin, increases food intake in healthy men." (Investigating the orexigenic effects)

After the endogenous peroxidase was inhibited with H 2 O 2 for 10 min at room temperature, the samples were incubated with 5% goat serum