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Alexa Fluor conjugated secondary antibodies were used to fluorescently label the antigens of interest and following dehydration in a series of increasing concentrations of methanol and cleared using dibenzyl ether, the membrane-bound slices were placed onto a glass microscope slide and imaged using a Zeiss LSM-800 inverted confocal microscope

Individuals with Vitamin B12 deficiency from dietary restrictions (vegetarian/vegan), gastrointestinal disorders, or certain medications benefit most

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However, pretreatment with 10 g/g GHK-Cu significantly increased both SOD activity and GSH levels, bringing them to values comparable to control mice