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Quantitative real-PCR Total RNA from cultured cells was extracted using TRIzol and ultra-micro nucleic acid-protein analyzer was used to measure concentration

increased the level of superoxide, hydrogen peroxide, and malondialdehyde

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[113] demonstrated that in the eyes of aged mice, the increased signaling of the STAT3 pathway induced the M2 polarization of macrophages, thus enhancing vascular proliferation and subsequently the formation of angiogenesis