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Description

Compounds were injected for 150 s, and the corresponding dissociation constants were measured for 300 s

The established maximum doses represent the upper limits studied in clinical trials, beyond which risk-benefit ratios become unfavorable

Cells were re-suspended in flow cytometry buffer (PBS, 0.5% BSA, 0.02% NaN3, 1 mM EDTA) and cell death and cell cycle phases were analyzed by flow cytometry in a BD FACSVerseTM flow cytometer (Becton Dickinson AG, Allschwil, Switzerland)