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The cDNA fragments were end-repaired, A-tailed, and ligated to adapters

Data from ERA5 (Hersbach et al., 2020) were used as input low-resolution data for our models and were downloaded from the Copernicus Climate Change Service ( Hersbach et al., 2023a, Hersbach et al., 2023b)

The reaction was quenched by addition of methanol (8.5 mL), N,N-dimethyl-ethylenediamine (3 mL, 27.2 mmol) was added and the solution was heated to reflux and stirred for three hours

Even so, the scientific evidence is still thin and the effect of B12 cream remains under question by many dermatologists, even though individual patients have reported successful results