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The intracellular amino acid level was calculated as follows

GSH regeneration is catalyzed by glutathione reductase using GSSG and NADPH generated by the pentose phosphate pathway (PPP, also called hexose monophosphate shunt, which also produces pentose phosphate sugars used in nucleotide synthesis.) The first reaction of the PPP, catalyzed by glucose 6-phosphate dehydrogenase (G6PD), is critical for producing an adequate amount of NADPH used in reductive biosynthetic pathways and importantly in the generation of GSH

Falade AO, Nwodo UU, Iweriebor BC, Green E, Mabinya LV, Okoh AI

Probably, if all cysteine residues were in the reduced form (with free sulfhydryl groups) high percentage of helical fragments would be possible