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Transforming growth factor suppresses glutamate-cysteine ligase gene expression and induces oxidative stress in a lung fibrosis model

In the latter case, cell-free supernatants were immediately frozen in liquid nitrogen and stored at 80 C, while the corresponding cell pellets were either extracted for total RNA or lysed for protein analysis

We're talking about molecular degradation from using the wrong diluent or, even worse, from aggressive shaking that can literally tear the delicate peptide structure apart through shear stress