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Nanoliposomes encapsulated rapamycin/resveratrol to induce apoptosis and ferroptosis for enhanced colorectal cancer therapy

Ann Oncol 24(5):1262-6, 2013

Labeled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 L of ice-cold PBS, and fixed by adding 700 L of ice-cold absolute ethanol while gently vortexing