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Research studies have used doses ranging from 10 mcg/kg to several milligrams daily, typically administered once or twice daily

COTI-2, A novel thiosemicarbazone derivative, exhibits antitumor activity in HNSCC through p53-dependent and -independent mechanisms

Cells were resuspended in a Medium Salt Lysis Buffer (20 mM Tris-HCl pH 8.0, 300 mM NaCl, 0.2% Triton X-100, 1 mM PMSF, and 3 mM 2-mercaptoethanol) plus protease inhibitor, and after sonication, the soluble fraction was bound to glutathione beads (GenScript)

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