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Purification of His-tagged proteins For protein expression, 400 mL of bacterial cultures (BL21 Codon Plus RIPL, Agilent Technologies, #230280) in lysogeny broth (LB)-medium (supplemented with 50 g per mL Amp and 34 g per mL Cam) were induced with 0.1 mM IPTG at an OD 600 of 0.81.0

HPRL causes hypogonadism by suppressing gonadotropin-releasing hormone, resulting in menstrual irregularities, infertility, and decreased libido in women

Glutathione consists of three amino acids: cysteine, glutamate, and glycine

Dose: 1mg to 2mg daily Frequency: Once daily, typically morning Administration: Subcutaneous injection Duration: 30 days on, 14 days off The cycling pattern prevents potential receptor desensitization and gives the body periodic breaks from exogenous copper peptide input