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Lower doses waste peptide through degradation

The reaction was performed in 200 L total volume at 42 C in 0.1 M MOPS-KOH buffer pH 6.5, 0.6 mM NADH, 1 mM PEP, 5 mM ATP, 5 mM MgCl 2 , 5.5 U LDH, 2.8 U PK, 0.4 U Saci_2033, using 20 L (diluted) samples with a glycerol concentration of at most 0.5 mM