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However, one of the primary drawbacks of OOAC models is the limited number of cells that can be cultured within these small devices that often are too minute for traditional biochemical detection methods such as Western blotting, gel electrophoresis, and colorimetric detection [225]

The threshold where ROS generation surpasses cellular antioxidant capacity culminates in cellular architecture erosion, underscoring the incipient demise (Qin Y