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The blot was blocked with 2.5% nonfat milk in PBS for a minimum of 1 h

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This process triggers inflammation, apoptosis, DNA damage, lipid peroxidation, and mitochondrial dysfunction, contributing to disorders, including metabolic cardiovascular diseases, Alzheimers and Parkinsons diseases, premature aging, and certain cancers (Kozarski et al

HA-MTX conjugate is expected to exhibit joint tropism because of selective mutual action with overexpressed CD44 receptors on activated macrophages and synovial cells