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P values and the number and type of replicates (designated n ) are provided in the relevant legends of the main figures, extended data figures and supplementary figures

Correct mutations and sequences were confirmed by sequencing both strands

General MTHFR Symptoms Beyond the systems above, many people with an MTHFR gene mutation report broader symptoms linked to inflammation, detoxification issues, and low B-vitamin activity: Chronic fatigue Eczema, asthma, hives, and allergies Chronic inflammation or pain Low immunity and frequent illness High homocysteine, low B12, or elevated liver enzymes Medications That Can Worsen MTHFR Gene Symptoms Certain drugs deplete folate or B12, reducing your bodys methylation capacity and aggravating MTHFR mutation symptoms

Scheltens, P