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Overall, the above described model systems fulfill complementary roles: HMC3/CHME-5 provides the most accessible, reproducible, and experimentally tractable platform for discovery-based and high-throughput applications, whereas iPSC-derived microglia offer superior translational relevance for patient-centered disease modeling and mechanistic validation

Future research should use mitochondria as a focus to explore the mechanisms of cuproptosis in SAW, particularly concerning mitochondrial oxidative stress, energy metabolism, and the TCA cycle

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