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The ATP7B-KO cell line with stable PRNP knockdown (ATP7B-KO:PRNP-KD) was generated by infecting ATP7B-KO cells with pLKO1-puro-CMV-tGFP shPRNP-lentiviral particles (Mission lentiviral transduction particles, SigmaAldrich) and upon selection with puromycin

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Much of the strongest data reports biochemical or histological outputs hydroxyproline content, collagen and glycosaminoglycan mass, gene-expression signatures

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