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Characterization of internet-purchased research peptides using LC-MS/MS and comparison to label claims

Synthesis of ( R )-1D228 (1) Preparation of the intermediate 3 : an aqueous solution of sodium carbonate (sodium carbonate 13.2 mmol, 1.35 g, 6.5 mL water) was added into a solution of 5-fluoro-2-chloropyrimidine (6.6 mmol, 0.87 g) in toluene (13 mL), into which were then added bis(triphenyl phosphine)palladium chloride (1.36 mmol, 0.096 g), acetyl phenyl boronic acid (6.5 mmol, 1.05 g), and ethanol (6.5 mL)

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The original rationale for studying the gastric pentadecapeptide was cytoprotection of gastrointestinal mucosa