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A 498 bp DIG labeled probe, which hybridizes 5 upstream of the replacement vector, was generated by PCR using 5 forward primer CTTACAGCATGGGCAGCAGACTC and 5 reverse primer GTCTCCTCCACGGGGGTCAG and Roche labeling kit (catalog 11636090910)

A non-targeting siRNA was used as a control

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Mannitol decoration facilitated brain delivery through mannitol-mediated endothelial interactions and liposomal fusion, thereby enhancing transport across the bloodbrain barrier