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The EV strain grew slightly faster with a maximum growth rate of 0.52 h 1 and an average final OD 600 of 17.3, whereas the TML2Car strain grew at a rate of 0.46 h 1 to an average OD 600 of 15.3 at the end of the fermentation after 48 h, with all strains converting 50% of the provided carbon source glycerol

Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) (Cat# C1005, Beyotime, China), and the plates were sealed with anti-fluorescence quenching solution (Cat# P0126, Beyotime, China) before imaging

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Advancements for targeting energy metabolism for disease intervention Energy metabolism is essential for maintaining cell viability, converting nutrients into energy, and ensuring their efficient use within the cell