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Although we used a mild STZ-induced diabetic mouse model that mimics certain aspects of type 2 diabetes, the inclusion of additional insulin-resistant or diet-induced models in future studies could enhance generalizability

Resulted homogenate was centrifuged 4000 g/15 min at 4 C and the amount of proteins was assessed with BCA kit (Thermo Fisher Scientific, #23225) in supernatant containing cytosolic fraction

5 Interactions between three metabolism-linked cell death pathways Ferroptosis, cuproptosis, and disulfidptosis are programmed cell death pathways triggered by metabolic stress

Unbiased sub-clustering of these cells revealed seven distinct cell types (Figs