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Losing the glutamate at position 3 sharply reduces its binding to IGF-binding proteins, which is why in cell culture it is reported to be roughly ten times more potent than intact IGF-1 at driving cell proliferation and hypertrophy [1]

Cells were then transferred to ice, washed twice with cold Dulbeccos phosphate-buffered saline, and lysed in 180 l SDS Loading Dye (Cell Signaling Technologies, Danvers, MA)

For example, cytomegalovirus (CMV) infection of the adult cochlea, unlike during the prenatal period, typically causes mild hearing loss and cochlear damage (Juanjuan et al., 2011

Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-1: potential role in idiopathic pulmonary fibrosis