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NOT 1 and Bam H1 (Fermentas, Burlington, Canada) restriction sites were introduced, which allowed for the insertion of the BDNF sequence downstream to the cytomegalovirus promoter in a pcDNA3.1(-) expression vector (Invitrogen, Carlsbad, CA, USA)

Its mechanisms of action have been primarily explored in non-clinical studies involving animal models, cell cultures, and limited human cohorts

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