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Cell lysates containing 25 g protein were incubated with 150 L of Master Mix (composed of 10x SOD buffer, WST-1 reagent, xanthine oxidase and H 2 O milliQ), 25 L of xanthine 1x and the absorbance was read at 450 nm in a BioTek Cytation 3 multiplate reader (BioTek Instruments Inc., USA)