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Manders overlap coefficient was primarily used, defined as i( R i G i )i R i 2 i G i 2 where R i is the intensity of the first fluorophore in an individual pixel and G i is the corresponding intensity for the second fluorophore in the same pixel

M.CabreraJ.et al (2025)

These innovations have the potential to enhance treatment efficacy, reduce side effects, and ultimately improve reproductive outcomes, especially in the context of aging and environmental challenges