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A cocktail of 11 sarbecovirus antigens and 1 control proteins (HIV-1 antigens), obtained commercially (SinoBiological) or internally produced (see below), spanning Spike S1 and S2 domains for SARS-CoV-2 or SARS-CoV-1 were covalently coupled to uniquely coded magnetic microspheres (Luminex) per manufacturers protocol and added to the plate in a final volume of 50 l/well

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