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Priolo C, Tang D, Brahamandan M, Benassi B, Sicinska E, Ogino S et al

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These metabolites stably bound to and activated the aryl hydrocarbon receptor, triggering downstream signaling that enhanced the expression of the tight junction protein ZO-1, thereby restoring intestinal barrier integrity

The SIF was a commercially available product formulated according to pharmacopeial guidelines, consisting primarily of potassium dihydrogen phosphate (KHPO), sodium hydroxide for pH adjustment, and purified water, without digestive enzymes