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For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent

Microb Biotechnol (2017) 10:105761

The efficiency of transduced iSPNs was calculated by counting cells showing colocalization of both tdTomato and EGFP (44.5% for the scrambled-shRNA and 47% for the Gsto2- shRNA) injected mice