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There are great concerns that many children in low and middle-income countries fail to reach their developmental potential due to poverty-related factors such as malnutrition, micronutrient deficiencies, parental illiteracy, and lack of stimulation and learning opportunities.47 Results from our study will provide a valuable contribution to the field of early risk and development in disadvantaged children and their families, and may also help to further integrate the Government of Nepal programme on the first 1000 golden days

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The second fully expanded leaves from the shoot apex were harvested at the same time of day to reduce circadian effects, immediately frozen in liquid nitrogen, and stored at 80 C until analysis

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