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The column was then washed with buffer A (300 L, 2,000 g , 2 min), and 10 mM aqueous NH 4 HCO 3 containing 5% CH 3 CN (300 L, 2000 g , 2 min), before peptides were eluted from the column as 15 fractions using 300 L buffer containing an increasing concentration of CH 3 CN in 10 mM NH 4 HCO 3 (%CH 3 CN = 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30, 35, 40, 45, 50, 75) (2000 g , 2 min)

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Growing evidence suggests that ferroptosis is present in various disorders, and it plays a very different role in different diseases (Liu et al., 2022)

Drug interactions and concurrent medications While AOD 9604 has not been shown to interact significantly with most medications, the research on drug interactions remains limited