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The study is open to adults at least 18 years of age who currently smoke ten or more cigarettes per day, intend to quit smoking, and have failed at least one previous attempt to stop smoking with or without therapeutic support

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HeLa and HEK-293T cells were maintained in either DMEM media (Gibco) containing 4.5g/L glucose, 110mg/L pyruvate, 4mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin or in RPMI media (Gibco) containing 2 mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin