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Mechanism separates the two camps as cleanly as the washout test does

healthcare professionals do not prescribe it, and its use falls outside established medical practice

After washing with TBST, cells were stained in a mixture of 1 g ml 1 Alexa Fluor 594 goat anti-rat IgG (HL), 1 g ml 1 Alexa Fluor 488 goat anti-rabbit IgG (HL) and Hoechst 33342 in Can Get Signal Solution 2 for 30 min at 25 C