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J Cell Physiol 202(3):642653 Petrini I, Giaccone G (2025) Developments of MEK inhibitors as future cancer therapies: what have we learned from preclinical and clinical studies

For flow cytometric analysis of aortic endothelial cells, digestion buffer consisted of 1X PBS containing DNase I (250 U/ml) and collagenase IV (2500 U/ml, LS004212, CellSystems, Troisdorf, Germany) and aortas were digested for 40 min under agitation at 37C

Fluorescence signals for A, Tau, Iba1, and GFAP were quantified as mean fluorescence intensity using ImageJ software (National Institutes of Health, Bethesda, MD, USA), with consistent threshold settings applied across all groups

The reaction was then cooled to 0 C and quenched by addition of a saturated aqueous solution of ammonium chloride (3 mL)