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Moreover, Real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis revealed a significant up-regulation in the mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor- (TNF-), inducible nitric oxide synthase (iNOS), interleukin-1 (IL-1), interleukin-6 (IL-6), and interleukin-12 (IL-12) in LPS-stimulated Raw264.7 cells (Fig