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For gene expression analysis, 1 g of total RNA for each sample was retrotranscribed into complementary DNA (cDNA) with the iScript TM cDNA Synthesis Kit (BIORAD, Hercules, CA, USA), following the manufacturer's instructions, using the GeneAmp PCR System 9700 (Perkin Elmer, Waltham, MA, USA)

6.1.4 Microsponges Microsponge-based carriers have been investigated as a potential platform for transdermal flavonoid delivery, providing a porous structure conducive to prolonged release and enhanced skin deposition ( in vitro release of 92.3% within 24 h

It has been observed that the apoptotic process in paclitaxel induced-painful neuropathy is due to the permeability transition pore opening, with the consequent cytochrome c release and Ca 2+ homeostasis dysregulation (Areti et al., 2014)