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Contrary to X-ray crystallography or electron microscopy, NMR spectroscopy does not provide direct information on the 3D structures, i.e., spatial position of atoms, but rather indirect measurements such as local conformations (e.g., from chemical shifts) or atom proximities in space

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Kinetic studies also confirmed that SIRT4 was the first mammalian sirtuin discovered to be physiologically regulated by the [NAD + ]/[NADH] instead of by [NAD + ] (Pannek et al., 2017) as 30 M NADH, a physiological concentration found in the mitochondrial matrix (Sallin et al., 2018), inhibited SIRT4 activity