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The membrane was blocked with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 h and then incubated with a primary antibody overnight at 4C

How I tested I spent four months testing water bottles across two testing cycles, trying out 53

To have a more general overview of the multiple functions of Dcr-2 in flies and mosquitoes, immunoprecipitation experiments were carried out and its protein interactome was determined by mass spectrometry (136, 139, 142)